I think you didn't get my point .IS there any way to connect to any bioinformatics webtool where i dont want to paste the sequence all the time and i can get the results by connecting my files to that webtool and get the results .As i have got 7000 sequence so that i can save my time not copying and pasting it all the time .is there any module available in BIOPERL for all this to do ???Where the sequences in my file can be connected and can be manipulated one by one ????