#!/usr/bin/perl
use warnings;
use strict;
use Tk;
use Tk::Pane;


my @List = (0..50);
my @cbvalue;
my @cbs; 
 
my $mw = tkinit;
$mw->geometry("200x200+100+100");

$mw->fontCreate('big',
   -weight=>'bold',
   -size=> 14
   );


my $button = $mw->Button(-text => 'show selected',
     -command => \&show_selected,
     -bg => 'yellow',
     -font => 'big',
         )->pack();

my $pane = $mw->Scrolled('Pane', 
-bg => 'white',
-scrollbars => 'osoe')->pack(-expand => 1, -fill => 'both');

foreach my $i ( 0 .. $#List ) {
    $cbs[$i] =  $pane->Checkbutton(
        -text     => "Number $List[$i]",
        -onvalue => 1,
        -offvalue => 0,
        -variable => \$cbvalue[$i],
        -font => 'big',
        -bg => 'lightseagreen',
    )->pack();

  $cbvalue[$i] = 0; #initialize selections to off

  }

MainLoop;

sub show_selected {
  my @selected;
      foreach my $i ( 0 .. $#List  ) {
        if ($cbvalue[$i] == 1){push @selected, $i} 
    }

print "selected: @selected\n";

}
[download]

Yes its possible, its trivial even, see the docs, and see/run the tk demo program "widget"

See also How do I post a question effectively?

perl -MTk -e " my $mw = tkinit; $mw->Checkbutton( -text => $_ )->pack for @ARGV; MainLoop; " milk milk lemonade