in reply to Re^2: calculation of charged amino acids
in thread calculation of charged amino acids

When I run that program on these fasta sequences:

>DROTME_HH_Q02936 
MRHIAHTQRCLSRLTSLVALLLIVLPMVFSPAHSCGPGRGLGRHRARNLY
PLVLKQTIPNLSEYTNSASGPLEGVIRRDSPKFKDLVPNYNRDILFRDE

>DROME_HH_Q02937 
MRHIAHTQRCLSRLTSLVALLLIVLPMVFSPAHSCGPGRGLGRHRARNLY
PLVLKQTIPNLSEYTNSASGPLEGVIRRDSPKFKDLVPNYNRDILFRDEE
GTGADRLMSKRCKEKLNVLAYSVMNEWPGIRLLTTTTTTESWDEDYHHGQ
YEGRAVTIATSDRDQSKYGMLARLAVEAGFDWVSYVSRRHIYCSVKSDSS
ESLH

>DROME_HH_Q02938 
GTGADRLMSKRCKEKLNVLAYSVMNEWPGIRLLTTTTTTESWDEDYHHGQ
YEGRAVTIATSDRDQSKYGMLARLAVEAGFDWVSYVSRRHIYCSVKSDSS
ESLH

I get this output:

Name: >DROME_HH_Q02937 
Number of acidic amino acids:33
Number of basic amino acids:35
Number of neutral amino acids:33

Name: >DROME_HH_Q02938 
Number of acidic amino acids:18
Number of basic amino acids:17
Number of neutral amino acids:18

Name: >DROTME_HH_Q02936 
Number of acidic amino acids:14
Number of basic amino acids:18
Number of neutral amino acids:14

The fasta tags for each protein must be unique.
  • Comment on Re^3: calculation of charged amino acids

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Re^4: calculation of charged amino acids
by yuvraj_ghaly (Sexton) on Jul 24, 2013 at 04:52 UTC

    are these fasta sequences in one file file or different???

    Paste all these sequences in one file, save in fasta format and then run the program. You will see the problem

      They're all in one file. I don't see the problem I'm afraid. If you like though, you could tell me what problem you're having.
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