This kind of "for" loop:
for (my $i=0; $i<=33; $i++) is not common in Perl. Try this:
#!/usr/bin/perl
use strict;
use warnings;
my $filename = '16lines.txt';
open (INFILE, '<', $filename) or die
"unable to read the file with 16+ lines $!";
my @lines = <INFILE>; #read all lines into an array
for my $file_no (0..33) #total 34 files?? Really? Maybe 0..31?
{
open (my $fh, ">", "$filename$file_no.vcf") or die
"problem opening $filename$file_no.vcf Oops $!";
print $fh grep{defined}@lines[0..15];
# the grep on defined lines is in case there are less
# 16 lines in the input file. I am sure there are other
# with perhaps split() to accomplish the same thing.
}
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