I would bet it's because one of your input lines doesn't have enough columns, and, in particular, doesn't have the
$colN-th column.
When $columns[$colN] is undefined, then trim returns undef which makes $NSC undefined causing the Uninitialized value in string eq error message.
To check this, right after spliting the columns just put in a check like:
...
while ($line = <ALL>) {
my @columns = split /,/, $line;
warn "column $colN not found" unless defined($columns[$colN]);
...
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