What is a nucleotide?
From
Bio::Seq
# gets sequence as a string from sequence object
$seqstr = $seqobj->seq(); # actual sequence as a string
$seqstr = $seqobj->subseq(10,50); # slice in biological coordina
+tes
$seqobj->length() # length
so I guess
$segstr = $segobj->subseg( $segobj->length - 20, $segobj->length );
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