It might be that tr (see Quote and Quote like Operators) will perform your reverse complement.

knoppix@Microknoppix:~$ perl -E '
> $seq = q{ACTGTGGCGTCAACTG};
> say $seq;
> ( $revSeq = $seq ) =~ tr{ATCG}{TAGC};
> say $revSeq;'
ACTGTGGCGTCAACTG
TGACACCGCAGTTGAC
knoppix@Microknoppix:~$

[download]

I hope this is helpful.

Cheers,

JohnGG

In reply to Re: Another Reverse Complement Question... by johngg
in thread Another Reverse Complement Question... by lkenefic

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