It's not clear what output you expect. To search for overlapping sequences, you can change the second group from non-grouping to a look-behind:
$sequence =~ /(ATG.*?(?<=TAA|TAG|TGA))/g

to get

ATGGTTTCTCCCATCTCTCCATCGGCATAA ATGA

It still extracts the shortest possible sequence for each starting point (so we lost the second output).

Update: It's possible to get all the sequences without experimental regex features and depending on the return value of print like here.

my @from; my $pos = -1; push @from, $pos while -1 != ($pos = index $sequence, 'ATG', $pos + 1) +; my @to; for my $end (qw( TAA TAG TGA )) { $pos = -1; push @to, $pos + 3 while -1 != ($pos = index $sequence, $end, $pos + + 1); } for my $f (@from) { for my $t (@to) { say substr $sequence, $f, $t - $f if $t > $f; } } __END__ Output: ATGGTTTCTCCCATCTCTCCATCGGCATAA ATGGTTTCTCCCATCTCTCCATCGGCATAAAAATACAGAATGATCTAA ATGGTTTCTCCCATCTCTCCATCGGCATAAAAATACAGAATGA ATGATCTAA ATGA
لսႽ† ᥲᥒ⚪⟊Ⴙᘓᖇ Ꮅᘓᖇ⎱ Ⴙᥲ𝇋ƙᘓᖇ

In reply to Re: Print A Sequence with Start codon and different Stop Codon by choroba
in thread Print A Sequence with Start codon and different Stop Codon by PerlKc

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