Likely, substr is all you need, if you already know what parts overlap and what parts do not overlap. Once you have the non-overlapping parts, you can go through them character by character and if they match, you output that character, and if they don't match, you output the pair. I think there can only be two pairs anyway, so a fancy trick would be to use binary XOR on the "nonmatched" parts:

use strict;

my @nonmatched = qw(TACTCAGGAG TACTCGGGAG);
my $difference = $nonmatched[0] ^ $nonmatched[ 1 ];

# Replace all zeroes (= matches) by the original string:
$difference =~ s/\0/substr($nonmatched[0],pos($difference),1)/ge;

# Replace the "weird" parts with the proper pairs
my %pairs = (
    'C' ^ 'T' => '(C,T)',
    'A' ^ 'G' => '(A,G)',
);

$difference =~ s/(.)/$pairs{$1} || $1/ge;

print $difference;
[download]

use 5.010;
use strict;
use warnings;
my $k = 25;
my %readrepo =(
    readA => "GCTGAGGCAGGAGAATTGCTTGAACCTGGGAGGCA",
    readB => "TACTCAGGAGGCTGAGGCAGGAGAATTGCTTGAAC",
    readC => "GCTGAGGCAGGAGAATTGCTTGAACTTAGGGGATG",   
    readD => "TACTCGGGAGGCTGAGGCAGGAGAATTGCTTGAAC",
);
    
my @readstoconcate = (
    "readA_1",
    "readB_2",
    "readC_1",
    "readD_2",
);
     
my @prefixes;
my @postfixes;
my $fixed;
    
foreach my $read (@readstoconcate) {
    my ($entry, $tag) = split '_', $read;
    if ($tag == 1) {
        push @postfixes, substr $readrepo{$entry}, $k;
        $fixed ||= substr $readrepo{$entry}, 0, $k;
    }
    else {
        my $k1 = length($readrepo{$entry}) - $k;
        push @prefixes, substr $readrepo{$entry}, 0, $k1;
        $fixed ||= substr $readrepo{$entry}, $k1;
    }
}
        
#
# Assume all entries in %readrepo are the same length.
#
foreach my $set (\@prefixes, \@postfixes) {
    next unless @$set;
    for (my $i = 0; $i < length $$set[0]; $i++) {  
        my $l = "\x0";
        my @c = grep {my $r = ($_ ne $l); $l = $_; $r}
                map {substr $_, $i, 1} @$set;
        local $" = ",";
        print @c == 1 ? $c[0] : "(@c)";
    }
}
continue {
    state $flag = 0;
    print $fixed unless $flag++;
}
print "\n";
    
__END__
TACTC(A,G)GGAGGCTGAGGCAGGAGAATTGCTTGAAC(C,T)T(G,A)GG(A,G)G(G,A)(C,T)(A
+,G)
[download]

This produces a slightly different output for your sample than you provided--but I think mine is right :)

(You'll have to explain to me why it isn't, if it isn't.)

This could probably be made quite a bit more efficient with further thought, but I wanted to check if it is correct first.

#! perl -slw
use strict;

my $k = 25;
my %repo = (
    "readA" => "GCTGAGGCAGGAGAATTGCTTGAACCTGGGAGGCA",
    "readB" => "TACTCAGGAGGCTGAGGCAGGAGAATTGCTTGAAC",
    "readC" => "GCTGAGGCAGGAGAATTGCTTGAACTTAGGGGATG",
    "readD" => "TACTCGGGAGGCTGAGGCAGGAGAATTGCTTGAAC",
);
my @order = ( "readA_1", "readB_2", "readC_1", "readD_2");

my( @heads, @tails, $common );
while( @order ) {
    my( $s1, $p1, $s2, $p2 ) = map split( '_', shift @order ), 1 .. 2;
    ( $s1, $s2 ) = ( $s2, $s1 ) if $p1 > $p2;
    push @heads, substr $repo{ $s2 }, 0, length( $repo{ $s2 } ) - $k;
    push @tails, substr $repo{ $s1 }, $k;
    $common = substr $repo{ $s1 }, -$k unless $common;
}

my $head = '';
for my $p ( 0 .. length( $heads[0] )-1 ) {
    my %uniq;
    ++$uniq{ substr $heads[ $_ ], $p, 1 } for 0 .. $#heads;
    if( keys %uniq > 1 ) {
        $head .= '(' . join( ',', keys %uniq ) . ')';
    }
    else {
        $head .= each %uniq;
    }
}

my $tail = '';
for my $p ( 0 .. length( $tails[0] )-1 ) {
    my %uniq;
    ++$uniq{ substr $tails[ $_ ], $p, 1 } for 0 .. $#tails;
    if( keys %uniq > 1 ) {
        $tail .= '(' . join( ',', keys %uniq ) . ')';
    }
    else {
        $tail .= each %uniq;
    }
}
print $head, $common, $tail;

__END__
c:\test>868716
TACTC(A,G)GGAGGAGAATTGCTTGAACCTGGGAGGCA(T,C)T(A,G)GG(A,G)G(A,G)(T,C)(A
+,G)
[download]

Dear BrowserUK,
I was trying the following input to your code above:

my $k = 15;
my %repo = (
    "readA" => "AAACATGAAAAGAAATGATGAAACAGA",
    "readB" => "AAATCATGAAACAGAGCCTCATCTCCC ",
);
my @order = (  "readB_1","readA_2");
[download]

Why it gives this output

AAACATGAAAAGGAGCCTCATCTCCC GCCTCATCTCCC
[download]

Instead of:

AAACATGAAAAGAAAT(G,C)ATGAAACAGAGCCTCATCTCCC
[download]

Following this alignment:

readA      AAACATGAAAAGAAATGATGAAACAGA
readB                  AAATCATGAAACAGAGCCTCATCTCCC
[download]

How can I fix it?


---
neversaint and everlastingly indebted.......

How can I fix it?

By giving better examples :) I had assumed on the basis of the sample supplied, that the overlapping sections were identical as in both cases supplied. I think javafans make that same assumption.

You'd need to accumulate the overlaps in a third array in the while, and then add another for loop to process them is the same way as @heads & @tails. (There obviously scope there for making the for loop a subroutine.

I'm done for today, but I'll be back tonight.

As I suggested:

#! perl -slw
use strict;
use Data::Dump qw[ pp ];

my $k = 15;
my %repo = (
    "readA" => "AAACATGAAAAGAAATGATGAAACAGA",
    "readB" => "AAATCATGAAACAGAGCCTCATCTCCC ",
);
my @order = (  "readB_1","readA_2");

#my $k = 25;
#my %repo = (
#    "readA" => "GCTGAGGCAGGAGAATTGCTTGAACCTGGGAGGCA",
#    "readB" => "TACTCAGGAGGCTGAGGCAGGAGAATTGCTTGAAC",
#    "readC" => "GCTGAGGCAGGAGAATTGCTTGAACTTAGGGGATG",
#    "readD" => "TACTCGGGAGGCTGAGGCAGGAGAATTGCTTGAAC",
#);
#my @order = ( "readA_1", "readB_2", "readC_1", "readD_2");

sub muster {
    local $^W;
    my( $bits ) = @_;
    my $muster = '';
    for my $p ( 0 .. length( $bits->[0] )-1 ) {
        my %uniq;
        ++$uniq{ substr $bits->[ $_ ], $p, 1 } for 0 .. $#$bits;
        if( keys %uniq > 1 ) {
            $muster .= '(' . join( ',', keys %uniq ) . ')';
        }
        else {
            $muster .= each %uniq;
        }
    }
    return $muster;
}

my( @heads, @bodys,  @tails );
while( @order ) {
    my( $s1, $p1, $s2, $p2 ) = map split( '_', shift @order ), 1 .. 2;
    ( $s1, $s2 ) = ( $s2, $s1 ) if $p1 > $p2;

    push @heads, substr $repo{ $s2 }, 0, length( $repo{ $s2 } ) - $k;
    push @bodys, substr $repo{ $s2 }, -$k;
    push @tails, substr $repo{ $s1 }, $k;
    push @bodys, substr $repo{ $s1 }, 0, $k;
}

my $head = muster( \@heads );
my $body = muster( \@bodys );
my $tail = muster( \@tails );

print $head, $body, $tail;

__END__
TACTC(A,G)GGAGGAGAATTGCTTGAACCTGGGAGGCA(T,C)T(A,G)GG(A,G)G(A,G)(T,C)(A
+,G)
TACTC(A,G)GGAGGCTGAGGCAGGAGAATTGCTTGAAC(T,C)T(A,G)GG(A,G)G(A,G)(T,C)(A
+,G)

AAACATGAAAAGAAAT(C,G)ATGAAACAGAGCCTCATCTCCC
AAACATGAAAAGAAAT(G,C)ATGAAACAGAGCCTCATCTCCC
[download]

---

Examine what is said, not who speaks -- Silence betokens consent -- Love the truth but pardon error.

"Science is about questioning the status quo. Questioning authority".

In the absence of evidence, opinion is indistinguishable from prejudice.

RIP an inspiration; A true Folk's Guy

I tried to google it, but without much success and I'm not proficient in the arts of bioinformatics.